Anti -Bacterial Activity of Punica granatum Flower Extract against Several Gram-Negative and Positive Bacteria

Background and objectives: Bacterial antibiotic resistance is a major issue in the process of infectious disease treatments. The aim of this study was an evaluation of the antibacterial activity of Punica granatum flower extract against several gram-negative and positive clinical bacterial isolates.   Methods: An adequate dried flower of an endemic mature Punica granatum plant was used for extraction. The standard strain of several gram negative and positive bacteria was chosen for this study, as well as some distinguished clinical strains such as Pseudomonas aeruginosa and Enterococcus spp. In order to indicate the antibacterial effect of Punica granatum mature flower, well-diffusion method was done for each bacterium of the extraction of the flower, so that zone inhibitions can be reported. MIC and MBC test was done.   Results: Disc diffusion test was done and the greatest zone inhibition Shigella was 39 mm and then Salmonella typhimurium 13.1 mm. The lowest antibacterial effect of P. granatum extraction was gained on Proteus with 6 mm of zone inhibition. The Highest MIC and MBC effect was obtained from antibacterial evaluation on S. typhimurium and S. epidermidis.   Conclusion: the antibacterial activities of medicinal plants, pharmaceutical companies are just using medicinal plants in association with synthetic drugs in order to obtain better results. Setting up a more analytic test on medicinal plants same as HPLC test could be the next stage of this study in order to reach to a higher reliance of medicinal plants antibacterial activities qualification So That we could combine them with synthetic drugs and improve their efficiency.